Table 1 Primer sequences used in RT-qPCR study
Gene | Forward (F) and reverse (R) primers (5′ ➔ 3′) | Amplicon size (bp) | Genome | Referencea |
|---|---|---|---|---|
CD44 | F: ACGAGGAGCAAAGCATGTGA R: GTGAGCCGTCCTCATTGTCA | 94 | A | [6] |
CD44 | F: CGGAGTACTGAGGGCATCAC R: TGACTGTTGTGATGATGGTGGT | 133 | B | this study |
ESR1 | F: CAGGCCTGCCGACTAAGAAA R: GGTCTTTCCGGATTCCACCT | 64 | A | this study |
ESR1 | F: CAGGCCTGCCGACTAAGAAA R: CTGGACTCCTGCTCCTCTCT | 119 | B | this study |
KRT5 | F: GGGTGTTGGAGCCGTGAGTGTC R: TGCCAAGACCACTGCCCATGC | 137 | A | [26] |
KRT14 | F: GCGAGGACGCCCACATCTCTTC R: TGAGCGCCATCTGCTCACGG | 150 | A | [26] |
LGR5 | F: GAAATGCTTTGATGGGCTCC R: TGATAGCAGTGGGGAACTCG | 80 | A | this study |
LGR5 | F: AACCAACTACGCCAGGTTCC R: CATCCAGGCGTAGAGACTGC | 70 | B | this study |
MSI1 | F: TTCGGGTTCGTCACGTTCAT R: TCGTTCGGGTCACCATCTTG | 139 | A | this study |
MSI1 | F: AGTACTTCAGCCAGTTCGGC R: CCTTCGGGTCAATCTGGATCT | 83 | B | this study |
NANOG | F: TGCACACCAGGCTTACAGCAGTG R: TGCTGGGTGTTGCAGCTTGTTC | 120 | A | [26] |
NANOG | F: TCTACCACAGAGCGGGTTTC R: CCCATTCCCGTAAGTCTGGC | 148 | B | this study |
OCLN | F: GAGGAGTGGGTGAAGAACGTG R: GGTGCCCGAGGGGTAGTA | 150 | A | this study |
OCLN | F: TCCCGGCTGCCATTTTAAGG R: GAACATGGTGAACCTCCGCC | 50 | B | this study |
OCT4/ cPOUV | F: TGCAATGCAGAGCAAGTGCTGG R: ACTGGGCTTCACACATTTGCGG | 114 | A | [26] |
OVAL | F: CGTTCAGCCTTGCCAGTAGA R: AGTATTCTGGCAGGATTGGGT | 60 | A | this study |
OVM | F: TATGCCAACACGACAAGCGA R: CCCCCTGCTCTACTTTGTGG | 133 | A | this study |
SOX9 | F: GAGGAAGTCGGTGAAGAACG R: GCTGATGCTGGAGGATGACT | 124 | A | [36] |
SOX9 | F: CAGCAAGAACAAACCCCACG R: TTCAACAGCCTCCACAGCTT | 147 | B | this study |
ACTB | F: CACAGATCATGTTTGAGACCTT R: CATCACAATACCAGTGGTACG | 101 | A | [37] |
UB | F: GGGATGCAGATCTTCGTGAAA R: CTTGCCAGCAAAGATCAACCTT | 147 | A | [38] |