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Fig. 8 | BMC Developmental Biology

Fig. 8

From: The skeletal ontogeny of Astatotilapia burtoni – a direct-developing model system for the evolution and development of the teleost body plan

Fig. 8

Development of the pectoral girdle of A. burtoni. a Visualization of ossification by fluorescent imaging of Alizarin red stained embryos. At 6 dpf and 7 dpf, only the cleithrum has ossified, followed by the fin rays and the post-cleithrum at 9 dpf. Embryos shown were also stained for Alcian blue, which in the fluorescent images shows cartilaginous elements as a dark counterstain. In the drawing these unossified endoskeletal girdle and fin elements are indicated in grey. Cb In situ hybridization for collagenX visualizes the ossifying dermal bones in the same sequence but approximately one day in advance of the mineralization process as detected by Alizarin red staining. Abbreviations: dpf: days post fertilization; AZR: Alizarin red

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