Fig. 2

pum1 transcripts mark the posterior end of O. dioica embryos. In situ hybridization analysis was performed with an antisense pum1 probe. The developmental stages included are: fertilized egg (a), 2-cell stage (b), 4-cell stage (c), 8-cell stage (d), 16-cell stage (e), and 32-cell stage embryos (f). pum1 transcripts are tightly localized to one side of the cell and found in close proximity to the plasma membrane. These transcripts are detected in the cell lineage which gives rise to the germ line (B4.1, B5.2) and the germ line blastomeres (B6.4) [9]. The white triangle points to the B6.4 cells, the PGCs, according to the fate map. Scale bar: 50 μm