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Fig. 1 | BMC Developmental Biology

Fig. 1

From: Expression and function of microRNA-9 in the mid-hindbrain area of embryonic chick

Fig. 1

MiR-9 expression pattern in chick neural tube. LNA-ISH staining of whole-mount chick embryos (a-c) and miR-9 activity indicator DFRS-S9 (d-k”) showed that miR-9 is active from around HH13 in chick neural tube. LNA-ISH against miR-9 at HH13 (a) revealed an initially weak expression from di- to rhombencephalon. At HH23 (b) miR-9 was strong in ventral hind- and midbrain and in diencephalon. At HH25 (c) it was also expressed weakly in forebrain and spinal cord. The miR-9 sensor DFRS-S9 showed overlapping GFP (d) and RFP (e) positive cells in the mesencephalon at HH10 (c). At HH14 (g-i) not all GFP positive midbrain cells (g) expressed also RFP (h, i). At HH17 (j-k”) many cells in the mesencephalon were positive only for GFP (j-k”), whereas, cells in the MHB still expressed RFP (k-K″) and therefore no miR-9. Abbreviations: Di-diencephalon, Mes-mesencephalon, MHB-mid-hindbrain boundary, Rh-rhombencephalon, Tel-telencephalon

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