Fig. 3

Egg stripping and microinjection. a Female Midas cichlid (here a golden morph of Amphilophus xiloaensis) with enlarged genital pore. b For in vitro fertilization, eggs are stripped from female fish into petri dishes. To fertilize the eggs, one or more males are stripped. Alternatively, eggs can be taken immediately after ‘natural fertilization). c The microinjection setup that is used for injecting the Midas cichlid embryos. d Orientation of eggs in custom-molded agarose injection plates. The eggs must be oriented in an upright position to allow injection precisely into or just below the cell. e Scheme of the construct used for the generation of the ubi::eGFP line