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Fig. 2 | BMC Developmental Biology

Fig. 2

From: Tol2 transposon-mediated transgenesis in the Midas cichlid (Amphilophus citrinellus) — towards understanding gene function and regulatory evolution in an ecological model system for rapid phenotypic diversification

Fig. 2

Experimental overview. a Midas cichlids are crossed. After successful fertilization, eggs are immediately collected. Alternatively, eggs can be fertilized in vitro. b Embryos at the one-cell stage are injected with a mix of Transposase mRNA, phenol red and a Tol2 flanked DNA-construct. c Positive embryos show a mosaic pattern of GFP fluorescence. They are screened and selected seven days after fertilization. GFP positive larvae are raised. d-e GFP positive individuals are crossed after 9–12 months (d) to obtain stable transgenic Midas cichlid lines (e)

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