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Fig. 4 | BMC Developmental Biology

Fig. 4

From: Respective contribution of the cephalic neural crest and mesoderm to SIX1-expressing head territories in the avian embryo

Fig. 4

SIX1 expression in quail-chick chimeras of the cephalic mesoderm. a Schematic representation of the grafting procedure. Part of the chick lateral paraxial mesoderm was unilaterally replaced by its quail counterpart (in red) at stage HH8, and the chimeras were analyzed at HH15 (red dotted lines: position and orientation of the sections shown in b-f and g-j). b-f and b’-f’(magnfications of boxed areas) At the level of the trigeminal ganglion (tg) and nerves, detected by HNK1 staining (e, f and e’), engrafted quail mesodermal cells, co-stained with SIX1 and QCPN, are found in the host lateral mesoderm near the trigeminal ganglion (arrows in c’, e’ and f’). g-j In the region of BA2, quail cells from the grafted mesoderm are present in the core mesenchyme of the BA, which strongly expresses SIX1. j’ Magnification of the boxed area indicated in j, showing grafted mesodermal cells that express both SIX1 and QCPN (arrows). D-V, dorso-ventral orientation. Scale bars, 50 μm

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