Table 1 Primers used for RT-qPCR and probe amplification. For dntm3aa, the same forward primer was used in both reactions
Target | Primer | Sequence (5′ → 3′) | Amplicon (bp) | Application |
|---|---|---|---|---|
dnmt1 | F | GCCGTCAAGTCACCGACCGCTCCTAA | 132 | qPCR |
R | CATTGACAAGATGGTTGGCTGTTTCCCA | |||
F | TGCACGTGTTTGCGCCCAGAGCTT | 669 | WISH | |
R | GAGCGCACTCCCTCACACTGACCACT | |||
dnmt3aa | F | CGCCCTCACAGCAACACACAGACCCT | 126 | qPCR |
R | CGATGACGAAGCCCCGCCCATCC | |||
R | CCGCACATGTAGCAGTTCCACGGGTCCTCT | 994 | WISH | |
dnmt3ab | F | TAGAGCCACCAGAGGAGGAGCGCAAT | 86 | qPCR |
R | GGGGCGTGTAGGCTGCCGCTT | |||
F | CATCATCCACCACCGCCACTGCCAC | 774 | WISH | |
R | TGCCGCTTCTGGCTCCACCCACATC | |||
dnmt3bb.1 | F | CGGACTGAACCCACACGCGCTCAACA | 93 | qPCR |
R | AGGACACGAGGACCACCAACGCACAG | |||
F | CGGACTGAACCCACACGCGCTCAACA | 963 | WISH | |
R | AGCAGAACCTCTCGACCACCGCAGCAA |