Table 1 Primer sequences
From: Cathepsin B inhibitor improves developmental competency and cryo-tolerance of in vitro ovine embryos
Gene symbol | Forward primer (5′-3′) | Reverse primer (5′-3′) | Annealing temp. (°C) |
|---|---|---|---|
BCL2 | CCTTCTTTGAGTTCGGAG | CCTTCAGAGACAGCCAG | 61 |
BAX | AGCGAGTGTCTGAAGCG | CCCAGTTGAAGTTGCCGT | 61 |
β-actin | CCATCGGCAATGAGCGGT | CGTGTTGGCGTAGAGGTC | 59 |