Fig. 4
From: Expression of Ciona intestinalis AOX causes male reproductive defects in Drosophila melanogaster
Individualized sperm cells remain lodged in the testes of AOX-expressing males. Fluorescence imaging of testes of 10-day old males of genotype X/Y; UAS-AOX F6/2; tubGS/DJ-GFP, under uninduced (upper panels) and mifepristone-induced (lower panels) conditions, was performed using phalloidin-TRITC to detect actin and the DJ-GFP fluorescence to detect the tails of elongated spermatids and mature spermatozoids. Note that in the proximal region of the testis (fluorescent confocal images, right, and red boxes on the white-light microscopy images, left) of the uninduced control sample (AOX-nonexpressor) only the bundles of not-yet-individualized elongated spermatids are observed, whereas in the same region in AOX-expressing males these cells appear individualized, tangled and/or disorganized, accounting for the accumulated whitish material shown in Fig. 2