Fig. 5
From: Pcsk5 is required in the early cranio-cardiac mesoderm for heart development
Nkx2.5Cre-driven deletion of Pcsk5. (a, a’) Transverse histological sections through the hearts of a control (Psck5 wt/flox ; Nkx2.5Cre) and Psck5 Δ1/flox ; Nkx2.5Cre embryos stained with hematoxylin and eosin. The mitral and tricuspid valves (MV, TV), right and left ventricles (RV, LV) atrium (RA) and left superior vena cava (LSVC) are indicated. Scale bars = 0.5 mm. (b) Structure (not to scale) of wildtype, floxed and conditionally deleted (Δ1) Pcsk5 alleles, showing position of exon 1 (ex1), loxP and frt sites, and genotyping primers: F1, R1 and R2. Product sizes are indicated. The floxed allele has a diagnostic 560 nucleotide PCR product whereas the D1 allele has a diagnostic 347 nucleotide PCR product. (c) Ethidium bromide stained agarose gel showing multiplex polymerase chain reaction products of allele-specific genotyping from embryonic hearts. Three hearts from each genotype were analysed at E15.5. The floxed allele is almost completely absent in the hearts of Cre-positive Pcsk5 Δ1/flox embryos