Fig. 3
From: Pcsk5 is required in the early cranio-cardiac mesoderm for heart development
External appearance, palatal and visceral anomalies in the Pcsk5; Sox2Cre conditional KO embryos. (a) External appearance of a control and Psck5 Δ1/flox; Sox2Cre (a’) embryos at E15.5. (a’) Psck5 Δ1/flox; Sox2Cre embryo is smaller than its control littermate, has oedema (Oe), hypoplastic hind limbs (HL) and exomphalos (Ex). The tail (T) is absent. (b’) MRI transverse section showing large palatal cleft in a Psck5 Δ1/flox; Sox2Cre embryo in comparison to its control littermate’s fully developed palate (P) (b). Tongue (T) and clivus (C) are shown. (c’) MRI transverse section through the Psck5 Δ1/flox; Sox2Cre embryo. The urinary bladder (UB) and the rectum (R) are disrupted by the presacral mass (PM). Spinal cord (SC) does not extend into the tail. (c) Correctly formed urinary bladder (UB), rectum (R) and tail (T) in a control embryo. (d’) Psck5 Δ1/flox; Sox2Cre embryo lacks kidneys although adrenal glands are presents; right adrenal gland (RAd), pancreas (P) and liver (Li) are indicated in the picture as well as right kidney (RK) in the control littermate (d). All scale bars = 0.5 mm