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Fig. 4 | BMC Developmental Biology

Fig. 4

From: Transcriptome profiling reveals expression signatures of cranial neural crest cells arising from different axial levels

Fig. 4

Validation of differentially expressed genes. a RT-PCR validation of genes significantly up regulated from r1-r2 and r4-derived NCCs. b-c Whole mount and sectioned in situ hybridisation of Nkx2.9 (b) and AnxA2 (c) validates RNAseq expression data. Nkx2.9 and is observed within the periocular mesenchyme (curved arrows) populated by r1-r2-derived NCCs. AnxA2 expression is observed within blood vessels and the second branchial arch which is populated by r4-derived NCCs (c, arrowhead). ov, otic vesicle; e, eye. Scale Bars = 500um

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BMC Developmental Biology

ISSN: 1471-213X

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