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Table 1 Hatching stimulants do not affect the pre-hatch development timeline of Heterodera glycines

From: Embryogenesis in the parasitic nematode Heterodera glycines is independent of host-derived hatching stimulation

Developmental stage

Water (n)

Zinc chloride (n)

Soybean root exudate (n)

3 cell

2.67 ± 1.15 (3)

3.24 ± 1.25 (4)

3.20 ± 1.3 (6)

4 cell

4.67 ± 2.08 (3)

5.33 ± 1.53 (3)

5.25 ± 0.96 (7)

Gastrula

75.71 ± 9.25 (6)

72.60 ± 1.51 (5)

70.60 ± 4.35 (7)

Tadpole

100.50 ± 8.31 (6)

97.25 ± 4.57 (4)

93.80 ± 5.77 (5)

J1 stage

112 ± 7.66 (7)

113.80 ± 9.49 (5)

107.72 ± 10.12 (10)

J2 stage

168.29 ± 7.16 (7)

165.20 ± 9.76 (5)

166.14 ± 10.16 (11)

  1. Fifteen synchronized two-celled eggs were placed in water, soybean root exudate or 3 mM ZnCl2 and examined at variable time points. Data are means ± s.d. of hours to reach a specific developmental stage following the two-cell stage. Numbers in parentheses indicate number of animals observed at that specific stage out of the fifteen. For some animals, observation of the specific stage was missed. The J2 stage was assessed by the presence of a fully formed stylet. No statistically significant differences were found among treatments