Fig. 4
DIC micrographs demonstrating that development of the stylet and cuticle in pre-hatched J2 Hetrodera glycines continues beyond the J1 to J2 molt. Pre-hatch J2s a immediately following the J1 to J2 molt retaining its old J1 cuticle (lower arrow) and without a completely developed stylet (upper arrow). Early pre-hatched J2s mechanically removed from the eggshell (b and d) show an incomplete stylet, including a complete lack of the stylet shaft and knobs b and a lack of longitudinal ridges (alae) along the lateral field of the cuticle d. In comparison, a fully formed J2 has a complete stylet c and well developed alae e. Scale, 15 μm