Fig. 5

Time-lapse imaging of the wing expansion process. a The time from eclosion until the beginning of expansion (45-60 minutes total) in both wild type and Cysu mutant wings. The same fly was photographed at the noted intervals. At around the time when wing expansion begins, the Cysu mutant wings fail to properly expand, resulting in a collapsed appearance. b Cell death occurs naturally in the wings following expansion. Red dots correspond to TUNEL-positive nuclei (arrows). Cell death occurs in the Cysu mutant wings as well. c The percentage of TUNEL-positive cells shows no significant increase in the mutant wings (n = 3) on the two-tailed t-test 95% confidence interval; P = 0.34