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Fig. 5 | BMC Developmental Biology

Fig. 5

From: Left-right axis asymmetry determining human Cryptic gene is transcriptionally repressed by Snail

Fig. 5

Interaction of Snail with the Cryptic-promoter in-vivo. Chromatin Immunoprecipitation (ChIP) was performed in PANC1 cells for the two putative Snail binding sites using a) semi-quantitative or b) qPCR. The cells expressing endogenous Snail were cross linked using formaldehyde followed by shearing and immunoprecipitation using a Snail specific or IgG control antibody. The resulting chromatin was reverse cross linked and amplified using the primers flanking the two putative Snail binding sites. Equal loading was confirmed by the amplification of input chromatin. The resulting blot (4A) and the quantification (4B) is representative of 3 experiments (n = 3)

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