Fig. 3

Endogenous Cryptic levels are attenuated by Snail expression and are restored upon Snail depletion in PANC1 cells. a Cryptic and Snail levels are measured by western blotting after transfecting different amounts of Snail/control/shRNA plasmids (2/4/6 μg of Snail plasmid and 4 μg of shRNA plasmid and total amount of plasmid made up to 8 μg with pCDNA3 empty vector). Equal loading is confirmed by beta-actin. The blot is representative of 3 experiments (n = 3). qPCR is performed on reverse transcribed samples to estimate the mRNA levels of (b) Snail and (c) Cryptic and is normalized to beta-actin expression (n = 4)