Fig. 2

Cryptic promoter activity in cells over expressing Snail. Plasmid construct expressing Snail was transfected in increasing concentrations (as indicated) in HEK 293 cells along with reporter constructs for Cryptic promoter activity by cloning the Cryptic promoter region upstream of the firefly luciferase gene. (a) The full length Cryptic promoter, (b) Promoter region containing a single Snail binding element (SBE1), and (c) deleted Snail binding elements are co-expressed with increasing concentrations of the vector expressing Snail. (d) Luciferase acivity is also measured for the Cryptic promoter construct either containing SBEI or SBEII mutant or full-length and for the vector alone. Empty reporter vector is used as vector control, pCDNA3 is used as control for Snail transfection and Beta-galactosidase construct is utilized to ensure equal transfection. The relative luciferase activity is plotted as a function of increasing Snail expression. Experiments are carried out in triplicates and repeated at least 3 times. Data with p < 0.05 is considered significant