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Fig. 5 | BMC Developmental Biology

Fig. 5

From: Generation and characterization of a novel transgenic mouse harboring conditional nuclear factor-kappa B/RelA knockout alleles

Fig. 5

Verification of RelA deletion via immunohistochemistry using antibodies targeting RelA N- and C-terminus. RelA+/+ and RelA-/- embryos, identified by PCR screen, were sectioned and immunostained for RelA with three different antibodies targeting different regions of RelA as depicted in the diagram. Robust immunostaining of RelA was observed with all three antibodies in the developing liver, brain and vertebrae of RelA+/+ embryos. In contrast, there was a complete absence of signal in RelA-/- embryos with antibodies targeting the C-terminus and a slight signal was observed in the liver with antibody targeting the N-terminus, possibly due to antibody cross-reactivity with other RHD containing proteins. This suggests that the recombined RelA alleles fail to produce RelA protein. N = 3 RelA+/+ and n = 3 Rel-/- embryo tissue sections were used in each experiment. Representative images from three experiments are shown. All images were captured at 600× magnification. Scale bar represents 50 microns

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