Fig. 6

Defects in hace1 morphants are partly rescued by coinjection of Rac1 MO. a Temporal expression patterns of Xenopus laevis rac1 and rac2. The expression levels of rac1 and rac2 were analyzed by qRT-PCR. Expression levels were normalized to those of Xenopus laevis ornithine decarboxylase 1 (odc1). b Immunoblot analysis shows that Rac1 MO inhibited translation of endogenous Rac1. Xenopus laevis embryos were injected with the indicated MOs (70 ng in total) and gfp mRNA (0.2 ng). α-tubulin was a loading control. c Coinjection of Rac1 MO (50 ng) and HACE1 MO (30 ng) led to milder developmental defects than did coinjection of control MO (50 ng) and HACE1 MO (30 ng). Anterior is to the left, and dorsal is to the top. d, e Embryos were classified into severe, mild, and normal groups on the basis of the extent of each defect. f, g The body length of embryos at stage 40-41 was quantified. Dorsal views of embryos are shown with anterior to the left. Values are expressed as the mean ± s.d. ***P < 0.001, unpaired t-test h Embryos injected with 30 pg of Rac1-V12 (constitutively active Rac1) mRNA and 170 pg of gfp mRNA displayed phenotypes similar to those of embryos injected with HACE1 MO (n = 38). Anterior is to the left, and dorsal is to the top