Fig. 7

Wnt9b, a UB and nephron regulator signal, is reduced in Wnt11 ^−/− kidneys. Kidneys were prepared from WT and Wnt11 ^−/− mice, sectioned and subjected to in situ hybridization with the Wnt9b probe. a–h Wnt9b, which is expressed in the UB and the collecting duct (CD) cells derived from it, was notably reduced in the case of Wnt11 knockout, especially in the CD cells situated in the subcortical region (compare f–g with b–c, arrows). Note the decreased expression of Wnt9b in the medullary CD as well (compare h with d). i–n Wnt9bremains reduced in the CD of the NB mouse kidney (compare m–n with j–k, arrows). Bars: a, e, i, l 500 μm; b, c, f, g 50 μm, d, h, j, k, m, n 100 μm