Fig. 4

Optical projection tomography shows Wnt11 dependent changes in the three-dimensional structure of the kidney. Kidneys of NB mice were dissected from the litters of Wnt11 ^+/− mating’s, processed and stained as whole-mount specimens with cytokeratin-Troma-1 antibody, and then subjected to optical projection tomography (OPT). The OPT data were analysed with 3D Imaris and Drishti software to examine the influence of Wnt11 knockout on the 3D layout of the kidney and its tubules. Troma-1 immunohistochemistry enables 3D presentation of the tubular projections in the kidney. Measurements of the Wnt11 ^−/− and WT kidney pelvis axes in the cortical-medullary/rostro-caudal (a–c) and dorsal-ventral axis (d–f) views. Wnt11 ^−/− kidney tubule reconstruction pointed to changes in the overall tubule convolution as compared with the controls. g–j One cortical-medullary tubule projection is highlighted in red in j, compare with (h). Bars: a, b, d, e, g, i 80 μm; h, j 300 μm