Table 1 Sequences of primers used for real time qPCR analyses
Target | Forward Primer | Reverse Primer |
|---|---|---|
β-actin | AAATCGTGCGTGACATCAAA | AAGGAAGGCTGGAAAAGAGC |
Ascl1 | ACTTGAACTCTATGGCGGGTT | CCAGTTGGTAAAGTCCAGCAG |
Brachyury | CTGGACTTCGTGACGGCTG | TGACTTTGCTGAAAGACACAGG |
Brg1 | CAAAGACAAGCATATCCTAGCCA | CACGTAGTGTGTGTTAAGGACC |
Flag-Gli2 S662A | GGACTACAAGGACGACGATGA | CAGAGGACAGGCCTTTTTCC |
Gata-4 | AAAACGGAAGCCCAAGAACCT | TGCTAGTGGCATTGCTGGAG |
Gli1 | CCAAGCCAACTTTATGTCAGGG | TCCTAAAGAAGGGCTCATGGTA |
Gli2 | CAACGCCTACTCTCCCAGAC | GAGCCTTGATGTACTGTACCAC |
Mef2c | TCTGTCTGGCTTCAACACTG | TGGTGGTACGGTCTCTAGGA |
Mesp1 | CATCGTTCCTGTACGCAGAA | TCTAGAAGAGCCAGCATGTCG |
Myhc6 | GGGACATTGGTGCCAAGAAGA | ATTGTGGATTGGCCACAGCG |
Myhc7 | ACTGTCAACACTAAGAGGGTCA | TTGGATGATTTGATCTTCCAGGG |
Nkx2-5 | AAGCAACAGCGGTACCTGTC | GCTGTCGCTTGCACTTGTAG |
Pax3 | TTTCACCTCAGGTAATGGGACT | GAACGTCCAAGGCTTACTTTGT |
Ptch1 | AAAGAACTGCGGCAAGTTTTTG | CTTCTCCTATCTTCTGACGGGT |
Tbx5 | CTTTCGGGGCAGTGATGAC | TTGGATGAGGTGGAGAGAGC |