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Fig. 1 | BMC Developmental Biology

Fig. 1

From: BRG1 interacts with GLI2 and binds Mef2c gene in a hedgehog signalling dependent manner during in vitro cardiomyogenesis

Fig. 1

Overexpression of GLI2 protein in mES[GLI2] cultures is maintained during differentiation. a Total protein extracts from corresponding differentiating cells were analyzed using immunoblot with GLI2 antibodies, with or without prior FLAG-IP. α-tubulin was used as a loading control. Relative band densities are listed below blots. The size of the upper band in the total protein blots ~180 kDa, is consistent with other studies [33, 67] and equivalent to the single GLI2 band that was pulled down by FLAG-IP. ( ) The non-specific binding seen below the GLI2 band is typical of this antibody [33, 47, 67]. b Transcription levels of the indicated genes in differentiating mES[Ctrl] (white bars) and mES[Gli2] (grey bars) cells were quantified using qPCR. Expression levels were normalized to β-actin, calibrated to day 0 mES[Ctrl] culture expression levels, and presented as a percentage of the highest expression level recorded, per gene. Error bars represent +/- SEM. The number of biological replicates analyzed (n) is indicated beside each graph. (†) n = 4 for total Gli2 days 0 and 4. One-tailed Student’s T-tests were used for statistical analyses. Grey lines represent paired T-tests; black lines represent unpaired T-tests; ( ) p < 0.05

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