Fig. 4
From: Efficient TALEN-mediated myostatin gene editing in goats
Myofibers and western blot analysis of MSTN-targeted goats. a Myostatin protein expression of K179, K52-1, and K52-2 was normalized to β-actin expression using western blot assays. b Muscle cross-sections of K52-2/MSTN +/− and K52-1/MSTN +/+. Tissue sections were prepared from the fore limb tricep muscles that was dissected from the cloned goat 2 h after birth. c, d Mean average area and frequency distribution of the myofibers in a given cross-sectional area. In the K52-2/MSTN +/− and K52-1/MSTN +/+ groups, 338–374 randomly selected myofibers was measured and used for analysis. *P value less than 0.05 considered as statistically significant. d The blue shaded area shows that more myofibers are in the 1000–3999 μm2 range in K52-1/MSTN +/+; the red shaded area shows that more myofibers are in the 4000–6999 μm2 range in K52-2/MSTN +/−