Fig. 1

Design of the TALENs assay targeting the goat MSTN locus. a Schematic showing the DNA-binding sites and spacer sequence of MTAL-2 in MSTN exon 1. b Modification of MSTN in GFFCs when transfected with one pair of functional TALENs (MTAL-2). Surveyor nuclease cleavage of the unsorted cells’ PCR products produced an expected 444-bp band, while WT cells produced a 289-bp band and a 145-bp band. ‘- -’ means no additional band was observed; ‘+ +’ means the additional band was observed. c Mutation identified in mixed transfection cells. DNA fragments surrounding the TALEN target sites were analyzed by direct DNA sequencing. The sequencing ideogram shows a series of peaks after the targeting site