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Fig. 2 | BMC Developmental Biology

Fig. 2

From: Relating proton pumps with gap junctions: colocalization of ductin, the channel-forming subunit c of V-ATPase, with subunit a and with innexins 2 and 3 during Drosophila oogenesis

Fig. 2

Ductin and V-ATPase subunit a are partly colocalized in plasma membranes and in the cytoplasm of follicle cells and germ-line cells, often in cytoplasmic particles or vesicles. a to c: Ductin (subunit c); a’ to c’: V-ATPase subunit a; a” to c” and d to f: merged images, ductin (red), V-ATPase subunit a (green) and colocalization of both (yellow), WFM. Nuclei were stained with DAPI. No specific staining was obtained with NIS (cf. inset in Fig. 4b). In young previtellogenic follicles (a to a”, stage 4), ductin and V-ATPase subunit a co-occur in anterior and posterior polar cells (aPC, pPC), in lateral and apical follicle-cell membranes (aFCM), and in the cytoplasm of FC, nurse cells (NCN, NC nucleus) and the oocyte (Ooc). While cytoplasmic particles or vesicles containing predominantly V-ATPase subunit a can be observed (a’), ductin is enriched in stalk cells (SC). In older previtellogenic follicles (b to b”, stage 6), ductin and V-ATPase subunit a are enriched in aFCM. While ductin is found in higher amounts in pPC and SC, V-ATPase subunit a is found in higher amounts in the cytoplasm of FC, NC and Ooc (b’). In vitellogenic follicles (c to c”, stage 10a), ductin and V-ATPase subunit a are colocalized especially in columnar and squamous FC. While ductin is enriched in NC membranes (NCM), V-ATPase subunit a is enriched in border cells (BC). d: Ductin and V-ATPase subunit a are colocalized in particles or vesicles (arrowheads, yellow) in squamous FC (FCN, FC nucleus, stage 10b, surface view). e: Ductin and V-ATPase subunit a are colocalized in lateral FC membranes (FCM) as well as in the cytoplasm (yellow, stage 10b, surface view). Particles or vesicles containing V-ATPase subunit a are found in FCN (arrowheads, green). f: In older follicles (stage 12, surface view), ductin and V-ATPase subunit a are colocalized in particles or vesicles in most of the FC (arrowheads, yellow) while, in anterodorsal FC (adFC), predominantly V-ATPase subunit a is found in the cytoplasm and in the nuclei (green vesicles). Scale bars, 10 μm

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