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Fig. 1 | BMC Developmental Biology

Fig. 1

From: Relating proton pumps with gap junctions: colocalization of ductin, the channel-forming subunit c of V-ATPase, with subunit a and with innexins 2 and 3 during Drosophila oogenesis

Fig. 1

Ductin is localized stage-specifically in plasma membranes and cytoplasmic vesicles of follicle cells and germ-line cells. a: On immunoblots of ovary extracts using Anti-ductin, a band at 29 kDa (M r, putative dimer) and weak bands at about 16 kDa (putative monomer) and 45 kDa (putative trimer) are recognized. a’: No specific staining was obtained with NIS. b to g: LSM images; b: Ductin is found in plasma membranes and cytoplasmic particles or vesicles, especially in polar cells (PC, stage 5, optical median section). c: Ductin becomes increasingly localized to plasma membranes in nurse cells (NC) and follicle cells (FC, stage 9). d: Ductin is found in plasma membranes of centripetally migrating FC (cFC, stage 10b). e: Ductin is localized in lateral and apical FC membranes (aFCM), in the oolemma (Ool) and, most prominently, in PC and stalk cells (SC, stage 10b). f: Ductin is found in plasma membranes of squamous FC and NC (arrowheads) and around NC nuclei (NCN, stage 10b, optical tangential section). g: Ductin is concentrated in plaques in lateral FC membranes (arrowheads, stage 9, optical tangential section)

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BMC Developmental Biology

ISSN: 1471-213X

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