Table 2 List of quantity and materials used for building the OpenSPIM

Laser2000

 Stradus VersaLase™ VersaLase 488/561

 Heat sink (special modification)

Pieter Fourie Design and Engineering CC

 2x RC1 vertical slit stilt

 11x RC1 Ø1/2" lens stilt

 3x Metal objective holder ring

 1x Detection axis holder, base

 1x Detection axis holder, top

 1x Infinity space tube

 2x Ø1"/Ø25.4 mm microscopy fluorescence emission filter holder, base

 2x Ø1"/Ø25.4 mm microscopy fluorescence emission filter holder, top

 8x RAIL CARRIER 15.4 mm, MOD ONLY

 1x Acrylic sample chamber T, OLYMPUS

 1x Metal chamber holder T, OLYMPUS

 8x INSERT FOR RAIL CARRIER 15.4 mm (RC1 MODIFIED)

 2x RC1 MOD, Ø1/2" lens stilt

 2x RC1 Iris stilt

 5x RC1 Ø1/2" mirror stilt

AHF Fluorescent filters

 1x F72-866; 446/523/600/677 HC Quadband Filter (Emission Filter)

 1x F59-486; Dual Line Laser Clean-up ZET 488/561

Picard Industries

 1x USB-4D-STAGE

Thorlabs parts

 2x DG05-1500-H1-MD; Ø1/2" SM05-Mounted Frosted Glass Alignment Disk w/Ø1 mm Hole

 2x NE20A-A; Ø25 mm AR-Coated Absorptive Neutral Density Filter, SM1-Threaded Mount, 350-700 nm, OD: 2.0

 5x TRF90/M; 90° Flip Mount for Ø1" Filters and Optics, Metric

 2x VA100/M; Adjustable Mechanical Slit, Metric

 10x LMR05/M; Lens Mount for Ø1/2" Optics, One Retaining Ring Included, M4 Tap

 5x KM05/M; Kinematic Mount for Ø12.7 mm Optics, Metric

 2x GM100/M; Ø25.4 mm Gimbal Mirror Mount, Metric, One Retaining Ring Included

 2x RSP1X15/M; Metric Rotation Mount, 360° Continuous or 15° Indexed Rotation

 2x BB1-E02; Ø1" Broadband Dielectric Mirror, 400-750 nm

 5x BB05-E02; Ø1/2" Broadband Dielectric Mirror, 400-750 nm

 2x AC127-050-A-ML; f = 50 mm, Ø1/2" Achromatic Doublet, SM05-Threaded Mount, ARC: 400-700 nm

 2x AC127-025-A-ML; f = 25 mm, Ø1/2" Achromatic Doublet, SM05-Threaded Mount, ARC: 400-700 nm

 2x AC127-019-A-ML; f = 19 mm, Ø1/2" Achromatic Doublet, SM05-Threaded Mount, ARC: 400-700 nm

 2x AC127-075-A-ML; f = 75 mm, Ø1/2" Achromatic Doublet, SM05-Threaded Mount, ARC: 400-700 nm

 2x ACY254-050-A; f = 50 mm, Ø1" Cylindrical Achromat, AR Coating: 350 - 700 nm

 24x RC1; Rail Carrier, 1" x 1", 1/4" (M6) Counterbored Mounting Hole

 2x LMR1/M; Lens Mount for Ø1" Optics, One Retaining Ring Included, M4 Tap

 2x SM1D12D; Ring-Activated SM1 Iris Diaphragm

 1x MB6090/M; Aluminum Breadboard, 600 mm x 900 mm x 12.7 mm, M6 Taps

 3x AV2/M; Sorbothane Feet, M6 Thread, 20 - 32 kg (44 - 70.4 lb) Load, 4 Pieces

 3x RLA300/M; Dovetail Optical Rail, 300 mm, Metric

 5x RLA150/M; Dovetail Optical Rail, 150 mm, Metric

 2x HW-KIT1/M; M4 Cap Screw and Hardware Kit

 1x HW-KIT2/M; M6 Cap Screw and Hardware Kit

 1x SPW602; SM1 Spanner Wrench, Graduated, Length = 3.88"

 1x BS004; 50:50 Non-Polarizing Beamsplitter Cube, 400 - 700 nm, 1/2"

 1x BS127CAM; 12.7 mm (0.50") Beamsplitter Cube Adapter for Compact 30 mm Cage Cube

 1x CM1-4ER/M; Compact Clamping 4-Port Prism/Mirror 30 mm Cage Cube, M4 Tap

 3x CL3/M; Compact Variable Height Clamp, M6 Tapped

 1x PH30/M; Post Holder with Spring-Loaded Hex-Locking Thumbscrew, L = 30 mm

 1x TR40/M; Ø12.7 mm x 40 mm Stainless Steel Optical Post, M4 Stud, M6-Tapped Hole

Olympus

 2x N2667500; UMPLFLN10XW objective (N.A. 0.30)

 1x N2667700; LUMPLFLN40XW objective (N.A. 0.80)

Video camera mounts & adapters

 1x U-TLU single port tube with lens

 1x U-TV1x video camera adapter (projection lens)

 1x U-CMAD3 video camera mount adapter

Andor

 1x Camera Zyla 5.5 3 Tap ex-demo model

ESImaging

 1x ESio TTL Controller

Misco.co.uk

 1x LN47340; Drobo 5D 5 Bays DAS Thunderbold x2 (10Gbs x2)

 5x LN46168; Red WD30EFRX 3 TB HDD