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Table 2 List of quantity and materials used for building the OpenSPIM

From: Light-sheet microscopy for everyone? Experience of building an OpenSPIM to study flatworm development

Laser2000
 Stradus VersaLase™ VersaLase 488/561
 Heat sink (special modification)
Pieter Fourie Design and Engineering CC
 2x RC1 vertical slit stilt
 11x RC1 Ø1/2" lens stilt
 3x Metal objective holder ring
 1x Detection axis holder, base
 1x Detection axis holder, top
 1x Infinity space tube
 2x Ø1"/Ø25.4 mm microscopy fluorescence emission filter holder, base
 2x Ø1"/Ø25.4 mm microscopy fluorescence emission filter holder, top
 8x RAIL CARRIER 15.4 mm, MOD ONLY
 1x Acrylic sample chamber T, OLYMPUS
 1x Metal chamber holder T, OLYMPUS
 8x INSERT FOR RAIL CARRIER 15.4 mm (RC1 MODIFIED)
 2x RC1 MOD, Ø1/2" lens stilt
 2x RC1 Iris stilt
 5x RC1 Ø1/2" mirror stilt
AHF Fluorescent filters
 1x F72-866; 446/523/600/677 HC Quadband Filter (Emission Filter)
 1x F59-486; Dual Line Laser Clean-up ZET 488/561
Picard Industries
 1x USB-4D-STAGE
Thorlabs parts
 2x DG05-1500-H1-MD; Ø1/2" SM05-Mounted Frosted Glass Alignment Disk w/Ø1 mm Hole
 2x NE20A-A; Ø25 mm AR-Coated Absorptive Neutral Density Filter, SM1-Threaded Mount, 350-700 nm, OD: 2.0
 5x TRF90/M; 90° Flip Mount for Ø1" Filters and Optics, Metric
 2x VA100/M; Adjustable Mechanical Slit, Metric
 10x LMR05/M; Lens Mount for Ø1/2" Optics, One Retaining Ring Included, M4 Tap
 5x KM05/M; Kinematic Mount for Ø12.7 mm Optics, Metric
 2x GM100/M; Ø25.4 mm Gimbal Mirror Mount, Metric, One Retaining Ring Included
 2x RSP1X15/M; Metric Rotation Mount, 360° Continuous or 15° Indexed Rotation
 2x BB1-E02; Ø1" Broadband Dielectric Mirror, 400-750 nm
 5x BB05-E02; Ø1/2" Broadband Dielectric Mirror, 400-750 nm
 2x AC127-050-A-ML; f = 50 mm, Ø1/2" Achromatic Doublet, SM05-Threaded Mount, ARC: 400-700 nm
 2x AC127-025-A-ML; f = 25 mm, Ø1/2" Achromatic Doublet, SM05-Threaded Mount, ARC: 400-700 nm
 2x AC127-019-A-ML; f = 19 mm, Ø1/2" Achromatic Doublet, SM05-Threaded Mount, ARC: 400-700 nm
 2x AC127-075-A-ML; f = 75 mm, Ø1/2" Achromatic Doublet, SM05-Threaded Mount, ARC: 400-700 nm
 2x ACY254-050-A; f = 50 mm, Ø1" Cylindrical Achromat, AR Coating: 350 - 700 nm
 24x RC1; Rail Carrier, 1" x 1", 1/4" (M6) Counterbored Mounting Hole
 2x LMR1/M; Lens Mount for Ø1" Optics, One Retaining Ring Included, M4 Tap
 2x SM1D12D; Ring-Activated SM1 Iris Diaphragm
 1x MB6090/M; Aluminum Breadboard, 600 mm x 900 mm x 12.7 mm, M6 Taps
 3x AV2/M; Sorbothane Feet, M6 Thread, 20 - 32 kg (44 - 70.4 lb) Load, 4 Pieces
 3x RLA300/M; Dovetail Optical Rail, 300 mm, Metric
 5x RLA150/M; Dovetail Optical Rail, 150 mm, Metric
 2x HW-KIT1/M; M4 Cap Screw and Hardware Kit
 1x HW-KIT2/M; M6 Cap Screw and Hardware Kit
 1x SPW602; SM1 Spanner Wrench, Graduated, Length = 3.88"
 1x BS004; 50:50 Non-Polarizing Beamsplitter Cube, 400 - 700 nm, 1/2"
 1x BS127CAM; 12.7 mm (0.50") Beamsplitter Cube Adapter for Compact 30 mm Cage Cube
 1x CM1-4ER/M; Compact Clamping 4-Port Prism/Mirror 30 mm Cage Cube, M4 Tap
 3x CL3/M; Compact Variable Height Clamp, M6 Tapped
 1x PH30/M; Post Holder with Spring-Loaded Hex-Locking Thumbscrew, L = 30 mm
 1x TR40/M; Ø12.7 mm x 40 mm Stainless Steel Optical Post, M4 Stud, M6-Tapped Hole
Olympus
 2x N2667500; UMPLFLN10XW objective (N.A. 0.30)
 1x N2667700; LUMPLFLN40XW objective (N.A. 0.80)
Video camera mounts & adapters
 1x U-TLU single port tube with lens
 1x U-TV1x video camera adapter (projection lens)
 1x U-CMAD3 video camera mount adapter
Andor
 1x Camera Zyla 5.5 3 Tap ex-demo model
ESImaging
 1x ESio TTL Controller
Misco.co.uk
 1x LN47340; Drobo 5D 5 Bays DAS Thunderbold x2 (10Gbs x2)
 5x LN46168; Red WD30EFRX 3 TB HDD