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Fig. 5 | BMC Developmental Biology

Fig. 5

From: Light-sheet microscopy for everyone? Experience of building an OpenSPIM to study flatworm development

Fig. 5

A single and dual-sided illumination, imaging test showing maximum projections of nuclei stained with the nucleic acid marker SytoxGreen. a Maximum projections of embryonic nuclei using left illumination path of the OpenSPIM microscope, b Both illumination paths and c Right illumination paths respectively. Image stacks were acquired in the following order: left illumination, right illumination, dual-sided illumination (a following c following b). All stacks and related insets have been processed identically

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