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Fig. 4 | BMC Developmental Biology

Fig. 4

From: Light-sheet microscopy for everyone? Experience of building an OpenSPIM to study flatworm development

Fig. 4

The benefit gained by applying the multi-view deconvolution method (5-angles used) vs. simple maximum projections of an acquired stack (1-angle) on an early staged M. crozieri embryo. a Depth color coding (Fire) of all nuclei after maximum projection of a raw z-stack (1-angle) b Depth color coding (Fire) of all nuclei after a maximum projection of a raw z-stack (1-angle) c Maximum projection of all nuclei after multi-view deconvolution. Additionally all nuclei missing in the single angle maximum projection (from b) have been coloured in red

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