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Table 1 Lectin labelling of different cells and tissues in Macrostomum lignano

From: Adhesive organ regeneration in Macrostomum lignano

Lectin

Acronym

Number specimen

Overall

Epi-dermis

Adhesive glands

Frontal glands 1, 2, 4

Frontal glands 3

Pharyngeal glands

Testes

Developing eggs

Cement glands

Antrum

Prostate

Lens culinaris agglutinin

LCA

n = 25

 

+++a

         

Phaseolus vulgaris erythro agglutinin

PHA-E

n = 28

 

+++b

         

Phaseolus vulgaris leuco agglutinin

PHA-L

n = 27

 

+++b

         

Succinylated wheat germ agglutinin

sWGA

n = 17

    

+++

++

   

++

 

Griffonia (Bandeiraea) simplicifolia lectin I

GSL I

n = 23

   

+++

 

++

 

+++

 

+++

 

Soybean agglutinin

SBA

n = 45

   

+++

 

++

 

+++

+

+++

++

Peanut agglutinin

PNA

n = 48

  

+++

+++

 

+++

+

++

++

+++

 

Ricinus communis agglutinin

RCA

n = 24

++

 

+++

++

   

+++

 

+++

+++

Concanavaline A

Con A

n = 32

 

+++

         

Wheat germ agglutinin

WGA

n = 23

~

          

Sambucus nigra agglutinin

SNA

n = 16

~

          

Maackia amurensis lectin II

MAL II

n = 18

~

          

Dolichos bilforus agglutinin

DBA

n = 21

~

          

Sophora Japonica agglutinin

SJA

n = 20

~

          

Ulex europaeus agglutinin 1

UEA 1

n = 12

~

          

neg. control (without lectin)

 

n = 36

~

          
  1. +weak labelling,++ intermediate labelling, +++ strong labelling, ~ unspecific background
  2. aprobably epidermal cell junctions
  3. bglycocalyx of epidermal microvilli and modified microvilli of anchor cells