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Fig. 4 | BMC Developmental Biology

Fig. 4

From: Late stage definitive endodermal differentiation can be defined by Daf1 expression

Fig. 4

Both Daf1+/- could differentiate into pancreatic and intestinal fates. a A schematic drawing showing experimental design. DE was sorted on day 5. The sorted cells were plated onto MEF feeders and cultured with pancreatic and intestinal differentiation medium. Daf1- or Daf1 + DE cells differentiated into Pdx1/GFP+ cells after 4 days culture (b) and Cdx2+ cells after 5 days culture (c). b Arrows depict small colonies of Daf1 + DE-derived Pdx1/GFP+ cells. Numbers depict differentiation ratios: Daf1-DE-derived Pdx1/GFP+ cells; 10.9 %, Daf1 + DE-derived Pdx1/GFP+ cells; 12 % (day 9). c Numbers depict differentiation ratios: Daf1-DE-derived Cdx2+ cells; 18.3 %, Daf1 + DE-derived Cdx2+ cells; 17.1 % (day 10). Scale bar; 100 μm

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