Fig. 2

Analysis of the adult incisor phenotype. a Micro-CT analysis of mandibular incisors at 2 months after birth. Left to right: image from a stereomicroscope, three-dimensional image, and cross-section. b Micro-CT analysis of mandibular incisors at 3 months after birth. Left to right: image from a stereomicroscope, three-dimensional image, and cross-section. c Left to right: image from a stereomicroscope, three-dimensional image, and cross-section. d Upper images are from a stereomicroscope. Left image is the lingual side. Right image is the buccal side. Lower images show the cross-sectional area of mandibular supernumerary teeth (arrowheads) and mandibular incisors. a–d Scale bars: 1 mm. e, f Volume and cross-sectional area of adult mandibular incisors. Numbers indicate respective incisors. Data in (e) are the mean volume ± standard deviation (SD) of mandibular incisors at 2 months (left) or 3 months (right) after birth. Data in (f) are the mean cross-sectional area ± SD of mandibular incisors at 2 months (left) or 3 months (right) after birth. g, h Volume and cross-sectional area of mandibular incisors in the adult F₂ generation at 4 months after birth. Data in (g) are the mean volume ± SD of mandibular incisors. Data in (h) are the mean cross section ± SD of mandibular incisors. I Statistical analysis of cross-sectional area of mandibular supernumerary incisors in the adult F₂ generation at 1 month. Error bars: SDs. j Images of trimmed and extracted mandibular incisors in a, b, and c. Whole computed tomography images of a mouse skull bone and an incisor with soft tissue removed using INTAGE Realia are shown (a, b, and c). We opened the image in Volume Player software (KGT Inc., Tokyo, Japan) (d), created .bmp files, and opened the files in Image J software (e ). *P < 0.05, **P < 0.01, ***P < 0.001