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Table 1 Effect of tissue-specific down-regulation of Dop1R2. A series of GAL4 drivers was used to down-regulate Dop1R2 expression in specific tissue/cell types. The lethality observed when down-regulating expression ubiquitously was recapitulated only when using the P{GawB}332.2 driver, which expresses GAL4 in the salivary glands and amnioserosa. Semi-lethality was observed when using the P{GawB}17A and P{GawB}c729 drivers. All of the above mentioned drivers resulted in abnormal melanization and cuticle phenotypes

From: A critical role for the Drosophila dopamine D1-like receptor Dop1R2 at the onset of metamorphosis

Gal4 driver Symbol

Expression

Phenotype

P{Act5C-GAL4}17bFO1a,b,c

Ubiquitous

Lethal, melanization and wing defect (in escapers)

P{Act5C-GAL4}25FO1a,b,c,d

Ubiquitous

Lethal, melanization and wing defect (in escapers)

P{GawB}332.3a,b,c

Salivary glands, amnioserosa

Lethal, melanization and wing defect (in escapers)

P{GawB}17Aa

Salivary glands, glia, cardia

Semi-lethal

P{GawB}c729a

Salivary glands, female follicle cells, male accessory glands, testis sheath, cyst cells

Semi-lethal, melanization and wing defect (in escapers)

P{GawB}elav[C155]a

Pan-neuronal

WT

P{GawB}Tab2[201Y]a

Primarily in mushroom bodies

WT

P{GawB}c698aa

3IL CNS, not in discs

WT

P{Eip71CD-GAL4.657}TP1-1a

3IL brain and epidermis

WT

Bursicon-α- GAL4a,c

Bursicon-α positive cells

WT

P{Ccap-GAL4.P}16a,c

Crustacean cardioactive peptide-secreting cells

WT

P{GawB}30Aa

Imaginal discs

WT

P{GawB}l(2)T32T32a

Amnioserosa, larval brain, wing discs

WT

P{GawB}c381a

Amnioserosa, embryonic PNS - stage 14

WT

P{Sgs3-GAL4.PD}TP1a

Salivary glands

WT

P{Lsp2-GAL4.H}3a

3IL fat body

WT

P{drm-GAL4.7.1}1.1a

Gastrointestinal tract, malpighian tubules

WT

  1. All phenotypes were assessed on progeny that developed at 29 °C
  2. ain combination with lab generated UAS-dsDop1R2 line 1
  3. bin combination with lab generated UAS-dsDop1R2 line 2
  4. cin combination with UAS-dsDop1R2 VDRC stock FBst0460369
  5. din combination with UAS-dsDop1R2 VDRC stock FBst0477151
  6. WT wild-type phenotype
  7. 3IL third instar larva