Fig. 9
From: A critical role for the Drosophila dopamine D1-like receptor Dop1R2 at the onset of metamorphosis
Dop1R2 is expressed in prepupal salivary glands and brain. a RNA obtained from the brain and salivary glands of w1118 prepupae was reverse transcribed, and PCR was performed in triplicate using primer sets corresponding to Dop1R2-RB, Dop1R2-RA/C or Act5C (as a normalization control). Transcript variants A/C and B are detected in the brain, and transcript variant B is detected in the salivary glands (the presence of a low abundance Dop1R2-RA/C transcript in salivary glands cannot be excluded). b Quantification of transcript levels (ImageJ software). Expression is quantified as band intensity for three biological replicates of Dop1R2-RB, or Dop1R2-RA/C, normalized to Act5C