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Fig. 7 | BMC Developmental Biology

Fig. 7

From: A critical role for the Drosophila dopamine D1-like receptor Dop1R2 at the onset of metamorphosis

Fig. 7

Dop1R2 down-regulation using the P{GawB}332.3 driver leads to developmental arrest at the pharate adult stage. Flies that escape lethality display a melanization phenotype. a Expression of Dop1R2 RNAi construct under the control of the P{GawB}332.3 driver (GAL4 expressed in the salivary glands and amnioserosa), induces 98.4 % (line 1) and 99.2 % (line 2) lethality before eclosion. Survival of RNAi flies (genotype: w1118;UAS-dsDop1R2/P{GawB}332.3-GAL4) is expressed as percent control progeny (genotype: w1118;TM6B/GawB-GAL4). n = 208 (line 1), n = 124 (line 2). Crosses with line 1 were done in triplicate; line 2 was used as a confirmatory single experiment. Error bars indicate the Standard Error of the Mean (SEM). b Images of pharate adults dissected out of the pupal case suggest a poorly formed abdomen (arrows, lines 1 and line 2) or incomplete cuticle formation (arrows, line 2). c Analysis of progeny that were switched from 29 °C (high RNAi) to 25 °C (attenuated RNAi) on a defined day post egg laying. Transfer day and temperature shift, as well as corresponding developmental stage are defined along the x-axis. Percent of Dop1R2 RNAi (line 1) (genotype: w1118;UAS-dsDop1R2/P{GawB}332.3-GAL4) that emerge vs. controls (genotype: w1118;TM6B/GawB-GAL4). Dop1R2 RNAi flies reared at 29 °C throughout development fail to emerge as adults, while those transferred to 25 °C prior to L3 larval/prepupal stage show higher eclosion. When flies are transferred between these two temperatures at different stages of development, the time course of lethality is revealed. n = 543. Driver stock: P{GawB}332.3-GAL4 (FBst0005398). d Reduced cuticle melanization (arrows) is observed in dsDop1R2 adult males that escape lethality at 25 °C. L: larval instar

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