Fig. 4

Down-regulation of Dop1R2 around larval-to-pupal ecdysis leads to developmental arrest. a Schematic of the temperature shift assay. b Analysis of progeny that were switched from 29 °C (high RNAi) to 25 °C (attenuated RNAi) on a defined day post egg laying. n = 1194 (line 1), n = 1107 (line 2). c Analysis of progeny that were switched from 25 °C (attenuated RNAi) to 29 °C (high RNAi) on a defined day post egg laying. n = 1969 (line 1), n = 2212 (line 2). Transfer day and temperature shift, as well as corresponding developmental stage are indicated along the x-axis. Each graph shows the percent of Dop1R2 RNAi (line 1 or line 2) (genotype: w¹¹¹⁸;UAS-dsDop1R2/+;Act5C-GAL4/+) that emerge vs. controls (genotype: w¹¹¹⁸;UAS-dsDop1R2/+;TM6B/+). Dop1R2 RNAi flies reared at 29 °C fail to emerge as adults. Flies transferred at 25 °C prior to the L3 larval/prepupal stage show higher eclosion. The difference in intervals between (b) and (c) reflects the temperature effect on the length of the life cycle (i.e., the life cycle is shorter when the flies primarily develop at 29 °C, and longer when the flies primarily develop at 25 °C). For each temperature shifts shown in (b) or (c), line 1 and line 2 were each assessed in triplicate. L: larval instar. Error bars indicate the Standard Error of the Mean (SEM)