Fig. 2

Med12 depletion enhances NSC adhesion in a manner reversible by concurrent depletion of Sdc2. a mNS-5 NSCs infected with lentiviruses expressing non-specific (NS) control, Med12-, or Cdk8-specific shRNAs were harvested and incubated with Calcein-AM prior to seeding onto 96-well plates. After washing, adherent cells were detected by fluorometry. The percentage of adhesion was calculated by dividing the background-subtracted fluorescence of adherent cells by the total corrected fluorescence of cells added to each well and multiplying by 100. b RNA from mNS-5 NSCs infected with lentiviruses expressing NS control, Med12-, or Cdk8-specific shRNAs as indicated were used for RT-qPCR, mRNA levels for each gene were normalized to β-actin mRNA and expressed relative to their corresponding mRNA levels in NS control shRNA-expressing cells. c mNS-5 NSCs co-infected with lentiviruses expressing shRNAs specific for Med12 or the indicated cell adhesion genes were assayed for cell adhesion as described in (a). 7All data represent the mean +/− SEM of at least three independent experiments performed in triplicate. p values were calculated by Student’s t test