Fig. 8

Silencing of Atrophin inhibits muscle histolysis. a In controls during HE, DEOM1s (arrow, −2 h) disintegrated into sarcolytes (+5 h, arrows), revealing the DIOM1s (arrow head, +5 h) underneath. The more laterally located DEOM2s (*, +5 h) histolyzed later after HE. b Histolysis of Atro ^shRNA DEOM1s was delayed until early pupation (arrows, +5 h), resulting in intact muscles (arrows) at +5 h. Subsequently, DEOM1s in A3-A5 were destroyed. The DEOM1s in A2 persisted into adulthood, resulting in crosswise overlapping muscles at +55 h. Atro silencing led to transient formation of vacuoles (v) at +12 h. c Histolysis of control DEOM1s (white contours, compare to A, +5 h) was accompanied by chromatin (histone-mKO) condensation. Compare to myonuclei in DIOM1 (grey contour). d Delayed DEOM1 histolysis in A3 was accompanied by cell shrinkage and chromatin condensation, which did not occur in the corresponding muscle in A2. Time-lapse datasets were recorded at 25 °C