Fig. 7
From: Live imaging of muscle histolysis in Drosophila metamorphosis
DIOMs detach and round up in response to AMPKα silencing. a Panels show the remodeling of DIOMs in abdominal segments A2 and A3. Remodeling involves reduction in diameter (atrophy) and rotation of the muscle fiber. b Silencing of AMPKα caused the loss of tubular morphology in DIOMs (arrow) between +10 h to +22 h. From +30 h onwards, DIOMs rounded up to give rise to floating muscle spheroids (+37 h, see Video 3), indicating a loss of attachment. c AMPKα shRNA muscle spheroids displayed condensation of histone-mKO tagged myonuclei (arrow in b, +37 h). d In late pupation after +50 h, muscle spheroids showed a sudden disappearance of tau-GFP, while condensed nuclei could still be observed for another 2 to 5 h (arrow in b, +55 h)