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Fig. 5 | BMC Developmental Biology

Fig. 5

From: Live imaging of muscle histolysis in Drosophila metamorphosis

Fig. 5

Selected gene perturbation cause cell death of DIOMs during different periods of pupation. (a) We scored proportion of DIOM1s (#cells) in abdominal segments A2 to A4 (6 per pupae) of the indicated number of samples (#pupae). Error bars indicate 95 % confidence intervals of proportions. (b) Box-and-whisker plots show the distribution of time of death (TOD). Outer grey boxes show first quartiles, medians and third quartiles. Whiskers show minimum and maximum values. (*) symbols represent outliers. Inner blue boxes indicate 95 % confidence intervals of the median. Circles indicate population means. The onset of muscle degeneration was assessed based on the earliest discernible alterations in cell morphology, e.g. loss of tubular shape. Cell death statistics were scored based on time-lapse images recorded by confocal microscopy at 22 °C

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