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Fig. 3 | BMC Developmental Biology

Fig. 3

From: Live imaging of muscle histolysis in Drosophila metamorphosis

Fig. 3

DIOMs are not required for eclosion. (a) Eclosion rates were scored for the indicated number of samples n per genotype. Error bars indicate the 95 % confidence intervals of the proportions. (b-d) Images of late pupae (right column) and their cases after eclosion (left column) expressing the indicated RNAi constructs in muscles using the Mef2-Gal4 driver. Muscles were labelled with Mhc-tau-GFP (green). Shells were visualized by auto-fluorescence. (a) Arrow heads of a control pupa indicate DIOM1s. (b) Although overexpression of Cp1-shRNA induced elimination of all persistent muscles, the adult fly managed to eclose. An arrow head points at abdominal segment A2. (c) Similarly, the conversion of DIOMs to muscle spheroids (arrow head) and their subsequent elimination in response to AMPKα RNAi did affect the eclosion of the adult fly

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