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Fig. 1 | BMC Developmental Biology

Fig. 1

From: Live imaging of muscle histolysis in Drosophila metamorphosis

Fig. 1

Detection of muscle cell death phenotypes by macro zoom microscopy. a Anatomical drawings of the dorsal sides of a fly during prepupal and pupal stage illustrate the fates of two population of larval body wall muscles. While the apically located DEOMs (blue) are destroyed during and right after head eversion, the more basally located DIOMs (yellow) survive throughout pupation into adulthood. A1-A7 indicate the positions of abdominal segments. Panels (b-i) show dorsal views (anterior on the left) of a prepupa (b, c) and late pupae (d-i) expressing the MHC-tau-GFP reporter to label muscles that were imaged using a macro zoom microscope. The first five abdominal segments (A1-A5) are indicated. (b) In prepupae, live doomed muscles like a DEOM1 in A4 and persistent muscles like a DIOM1 in A3 can be viewed through transparent cuticles. (c) Close-up of (b). d In late pupation, persistent larval muscles that escaped histolysis and were remodeled are seen in A1-A5. The thorax contains the prominent indirect flight muscles (IFM). e RNAi of Rm62, f RNAi of Cp1 and g overexpression of dominant negative TOR (TOR TED) induced cell death of persistent muscles. h Atrophin (Atro) RNAi prevented histolysis of doomed DEOMs in the 2nd and, occasionally, 3rd abdominal segment. g AMPKα RNAi induced degeneration of DIOMs into muscle spheroids (arrow). None of the gene perturbation affected the development of newly formed adult muscles like the IFMs. The 500 μm bar corresponds to all panels except (c)

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