Skip to main content
Fig. 5 | BMC Developmental Biology

Fig. 5

From: Co-culture with neonatal cardiomyocytes enhances the proliferation of iPSC-derived cardiomyocytes via FAK/JNK signaling

Fig. 5

Focal adhesion kinase (FAK) and c-Jun N-terminal kinase (JNK) are involved in coculture-mediated myocardial differentiation. a, the phosphorylation of FAK and JNK were performed. b, cells were transfected with FAK siRNA or treated with specific JNK inhibitor (SP600125), the double immunostainings for cTnT (red) and CX43 (green) was examined, nuclei in the same field were stained with DAPI (blue). Note the presence of gap-junctions (immunostaining for Cx43, green) at the interphase (arrow heads) between the cells. c, West blot showed that the cells transfected with FAK siRNA expressed lower levels of FAK protein than controls. In addition, the p-JNK protein level was also significant decreased after transfected with FAK siRNA, compared to the control at the same time. Scale bar =100 μm, *: P < 0.05 versus control, #: P < 0.01 versus control

Back to article page