Fig. 4

Expression of cardiac specific proteins in induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) on day 12. a, double immunostaining of cells by antibodies against cTnT (red) and CX43 (green). b, double immunofluorescent staining for cTnT (red) and MLC-2 V (green), c, double immunofluorescent staining for cTnT (red) and α-actinin (green). Nuclei in the same field were stained with DAPI (blue). Merged figures were made by FV10-ASW Systems. d, growth curves of cells in co-culture and control analyzed by a MTT assay. The growth of the cells in co-culture was significantly better than that in control after day 6. Scale bar =20 μm,*: P < 0.05 versus control, **: P < 0.01 versus control