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Fig. 1 | BMC Developmental Biology

Fig. 1

From: Co-culture with neonatal cardiomyocytes enhances the proliferation of iPSC-derived cardiomyocytes via FAK/JNK signaling

Fig. 1

Myocardial differentiation of induced pluripotent stem cells (iPSCs) in the indirect co-culture model. a the culture of undifferentiated iPSC colonies with Oct4-GFP+ expression (green) on feeder cells. b The indirect co-culture system: a facile cell expansion and stem cell differentiation system with continuous medium conditioning while preventing mixing by hanging culture inserts. Two cell populations that are co-cultured in different compartments (insert and well) but can communicate via paracrine signaling through the pores of the membrane. c, d, e show the development of embryoid bodies (EBs) from 5 to 10 days in grown alone, co-culture with mouse embryo fibroblasts (MEFs), and co-culture with neonatal cardiomyocytes (NCMs) (c, 5-day-old EB cultured in suspension. d, 7-day-old EB. e, 10-day-old EB). Hanging inserts removed when photographed). There are few remaining GFP+ colonies (green) after 10 days. Scale bar =300 μm

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