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Fig. 4 | BMC Developmental Biology

Fig. 4

From: PfSMAD4 plays a role in biomineralization and can transduce bone morphogenetic protein-2 signals in the pearl oyster Pinctada fucata

Fig. 4

Knockdown of the PfSMAD4 gene by means of RNAi. a The expression levels of PfSMAD4 mRNA in the mantle were determined with qPCR 7 days after injection. Five oysters were used in each experiment. Statistically significant differences were analyzed by means of one-way analysis of variance. Asterisk indicates a significant reduction (P < 0.05) as compared with PBS-injected oysters. b and c SEM images of the surface of the nacreous layer of the oysters injected with PBS and 80 μg of PfSMAD4 dsRNA respectively

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