Table 1 Primers for genes whose expression was evaluated by the SYBR® Green method
Gene (GenBank ID) | F primer sequence (5′ to 3′) | R primer sequence (5′ to 3′) | Expected product size |
|---|---|---|---|
RFX6 (306518575) | TCTCTTTGACCAGCATGTCG | CTGTGCTGCCTGAAATGGTA | 104 bp spanning region within exon 12 |
CFTR (306514) | CTATGACCCGGATAACAAGGAGG | CAAAAATGGCTGGGTGTAGGA | 107 bp spanning region within exon 4 of all transcript variants |
CK19 (239735540) | TTTGAGACGGAACAGGCTCT | CTCGGCCATGACCTCATATT | 279 bp spanning region within exon 3 |
SOX9 (758102) | AAGCTCTGGAGACTTCTGAA | TAACGGGGCTCACGAGCGGC | 271 bp spanning exons 1 and 2 in the CDS |
RFX3 (Harvard PrimerBank ID 19743882c2) | CCAGGTGACTACCGTGGTCT | GCTGCTGATGAGTTGTCCTCC | 88 bp spanning region within exon 1 |